Clinical and Biochemical Effects of Repeated Injectable Platelet-Rich Fibrin Application in Periodontitis Treatment: A Randomized Controlled Clinical Trial
International Journal of Dental Hygiene, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2026
- Doi Numarası: 10.1111/idh.70162
- Dergi Adı: International Journal of Dental Hygiene
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CINAHL, EMBASE, MEDLINE, Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest)
- Anahtar Kelimeler: I-PRF, myeloperoxidase, PDGF-BB, periodontitis, platelet factor 4, TGF-beta 1
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objectives: This study aimed to investigate the clinical and biochemical effects of repeated injectable platelet-rich fibrin (i-PRF) application into periodontal pockets following scaling and root planing (SRP) in patients with periodontitis. Methods: Twenty patients with Stage III Grade B periodontitis were enrolled in this randomized, controlled, split-mouth study. i-PRF was applied into all periodontal pockets at the test site after SRP and repeated after 2 weeks, whereas the control site received only sterile saline irrigation. Clinical periodontal parameters were recorded at baseline and at 3 months. Gingival crevicular fluid (GCF) samples were collected at baseline, on Day 7 following each i-PRF application, and at 3 months. Levels of Platelet Factor-4 (PF4), Platelet-Derived Growth Factor-BB (PDGF-BB), Transforming Growth Factor-β1 (TGF-β1), and Myeloperoxidase (MPO) were analysed using the enzyme-linked immunosorbent assay (ELISA) method. Results: In periodontal pockets with an initial depth of ≥ 4 mm, no statistically significant differences were observed between the groups in probing depth (PD) or bleeding on probing (BOP) at 3 months (p > 0.05). However, clinical attachment loss (CAL), Plaque Index (PI), and Gingival Index (GI) values were significantly lower in the test sites than the control sites at 3 months (p < 0.01, p < 0.05, and p < 0.05, respectively). TGF-β1 total amounts were higher in the test sites at Week 2 and at Month 3 (p < 0.01). MPO total amounts were higher in the test sites at Week 4 compared to control sites (p < 0.05). Conclusions: Adjunctive use of i-PRF with SRP may enhance tissue healing and improve periodontal clinical parameters. Trial Registration: ClinicalTrials.gov NCT07029035, 19 June 2025 (retrospectively registered).