In vitro genotoxicity of calcium phosphate-based bone substitute material on human peripheral mononuclear leukocytes
Acta Medica Mediterranea, cilt.29, sa.4, ss.723-729, 2013 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 29 Sayı: 4
- Basım Tarihi: 2013
- Dergi Adı: Acta Medica Mediterranea
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.723-729
- Anahtar Kelimeler: Calcium phosphate-based bone substitute materials, calcium phosphate cements, DNA damage, comet assay, oxidative stress
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Background and aim: Calcium phosphate-based bone substitute materials, also called calcium phosphate cements (CPCs), are widely used in orthopedic surgery. However, the reported results regarding the genotoxicity using these materials are inadequate in literature. We evaluated the DNA damaging effects of a CPC on peripheral mononuclear leukocyte cultures. Material and methods: Mononuclear leukocytes from nine healthy donors blood samples were separated, and five culture plates were created from each donor's cells. Three were used to evaluate concentration-related effects (10, 100, and 200 μg/mL) of CPC and the others were used as negative and positive controls. DNA damage was assessed using the comet assay, and changes in oxidative status parameters, including total oxidant status (TOS) and total antioxidant capacity (TAC), were measured using the remaining culture supernatant. Results: We found that mononuclear leukocyte DNA damage increased with the concentration of CPC (p < 0.05). Relative to the negative control group, the highest mononuclear leukocyte DNA damage was found with 200 μg/mL CPC (p = 0.001). In addition, TOS and TAC values for the culture supernatants were not significantly different among the groups (p > 0.05). DNA damage was positively correlated with increasing concentration of CPC (r = 0.824; p < 0.001), but there was no significant correlation between DNA damage and oxidative status parameters within each group. Conclusions: This study indicates that the DNA-damaging effects of CPC may be due to a concentration-dependent direct chemical effect. Thus, when using CPCs in clinical practice, their potential to cause DNA damage should be considered.