Comparison of an electrochemical biosensor with optical devices for hemoglobin measurement in human whole blood samples
Clinica Chimica Acta, cilt.412, sa.23-24, ss.2150-2156, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 412 Sayı: 23-24
- Basım Tarihi: 2011
- Doi Numarası: 10.1016/j.cca.2011.07.026
- Dergi Adı: Clinica Chimica Acta
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.2150-2156
- Anahtar Kelimeler: Hemoglobin, Electrochemical, Biosensor, Capillary blood
- Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
Background: An electrochemical based biosensor for hemoglobin measurement was developed as an alternative to the traditional optical method, and underwent testing for use in professional settings. Methods: The affects of samples' freshness, hemolysis, bilirubin on the electrochemical method, as well as the repeatability, precision and accuracy were studied, using optical method devices as references. Results: Samples were stored at room temperature or in a cold environment for 7. days, partially or completely hemolyzed samples, and samples containing bilirubin with a concentration of up to 150. mg/l were investigated with no effects for interfering studies. Repeatability of finger blood testings was verified with six consecutive tests on nine volunteers, results ranged from 3% to 8% variation. The test results of BeneCheck were correlated with Sysmex, Beckman Coulters, Cell-Dyn and HemoCue methods, the results have shown similar and 95% of test results were within a ± 15% bias. Conclusions: BeneCheck hemoglobin test system performed well and accurately, while requiring 1μl of blood sample and 10. s detection time. Based on the cost, accuracy, sample volume, measuring time, ease of viewing and portability, BeneCheck deliver the best characteristics for these purposes. © 2011 Elsevier B.V.