Rosmarinic Acid Mitigates Oxidative–Inflammatory Injury in Rat Testicular Torsion– Detorsion Model


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GÜLER M. C., Baylan H., Erdoğan D. G., TANYELİ A., Eraslan E., EKİNCİ AKDEMİR F. N.

Sakarya Medical Journal, cilt.16, sa.1, ss.76-85, 2026 (Scopus, TRDizin)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Sayı: 1
  • Basım Tarihi: 2026
  • Doi Numarası: 10.31832/smj.1776904
  • Dergi Adı: Sakarya Medical Journal
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.76-85
  • Anahtar Kelimeler: Ischemia-reperfusion, Rat, Rosmarinic acid, Testicular torsion-detorsion
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Sağlık Bilimleri Üniversitesi Adresli: Hayır

Özet

Objective: We aimed to determine whether pre-detorsion rosmarinic acid (RA) mitigates oxidative and inflammatory damage in a rat torsion–detorsion (T/D)-induced I/R injury model. Methods: Thirty-two male rats were randomised (n=8/group) to sham, T/D, T/D + RA 40 mg/kg, or T/D + RA 80 mg/kg. T/D consisted of 2 h torsion followed by 2 h reperfusion. RA (i.p.) was administered 30 min before detorsion. Testis tissue was analysed for oxidative stress markers (MDA, MPO, OSI, TOS), antioxidant parameters (SOD, TAS), and inflammatory cytokines (TNF-α, IL-1β). Results: Compared with sham, T/D caused high MDA, MPO, TNF-α, TOS/OSI, IL-1β, and low SOD, TAS. RA significantly reversed these changes versus T/D, lowering oxidative and inflammatory markers and enhancing antioxidant defences. The high dose RA was equal or superior to low dose—with clearer gains for SOD, TAS, MPO, and cytokines—whereas TOS/OSI improvements were similar at both doses. Conclusions: RA attenuates oxidative and inflammatory injury in testicular T/D as a peri-detorsion adjunct.