The effects of thioredoxin and L-name on hepatic damage in the experimental liver ischemia/reperfusion model: Can a cumulative effect be obtained? Deneysel karaciǧer i̇skemi/reperfüzyon modelinde tiyoredoksin ve L-name'in karaciǧer hasari üzerine etkilerinin i̇ncelenmesi: Kümülatif etki elde edilebilirmi?
Turkish Journal of Biochemistry, cilt.35, sa.4, ss.300-306, 2010 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 35 Sayı: 4
- Basım Tarihi: 2010
- Dergi Adı: Turkish Journal of Biochemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.300-306
- Anahtar Kelimeler: Antioxidant system, Apoptosis, Ischemia/reperfusion, L-name, Nitric oxide, Reactive oxygen species, Thioredoxin
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objectives: The aim of this study was to evaluate the effects of thioredoxin and N-nitro-L-arginine methyl ester (L-NamE) on ischemia/reperfusion injury after partial liver ischemia was induced by selective clamping of the portal veins and hepatic arteries of rats. Material and Method: Rats were subjected to 1 hour of 70% hepatic ischemia, followed by reper-fusion or sham. at the end of ischemia, phosphate-buffered saline solution, L-NamE and thiore-doxin with or without L-NamE were infused. analysis was performed at pre-ischemia, onset of ischemia and postreperfusion. Hepatic tissue blood flow was evaluated by laser Doppler. afterwards, biochemical analyses were performed. Immunohistochemical method was used for apop-tosis evaluation. Results: Thioredoxin application increased hepatic tissue blood flow in reperfusion. after the inhibition of NOS enzyme with L-NamE, alanine aminotransferase, aspartate aminotransferase and P-selectin levels were significantly decreased when compared to ischemia/reperfusion group; this decrease was lower than the one in the thioredoxin group. Endothelins, nitric oxide, malondi-aldehyde levels and apoptosis ratio in the group in which L-NamE was applied had a considerabe extent of decrease when compared to ischemia/reperfusion group, and also it was significantly higher than the one observed in the thioredoxin group. Conclusion: Thioredoxin is one of the important anti-oxidants in ischemia/reperfusion injuries for reducing the damages, and that excess amount of nitric oxide depression creates protective effects. No significant difference was detected between values of thioredoxin and L-NamE administration, which means that the co-use of thioredoxin and L-NamE did not create a cumulative effect. © TurkJBiochem.com.