Investigation of the preventive effect of proanthocyanidin in ischemia-reperfusion injury in lung transplantation: An experimental study Akciger naklinde proantosiyanidinin iskemi-reperfüzyon hasarini önleyici etkisinin arastirilmasi: Deneysel çalisma
Turkish Journal of Thoracic and Cardiovascular Surgery, cilt.26, sa.4, ss.606-613, 2018 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 26 Sayı: 4
- Basım Tarihi: 2018
- Doi Numarası: 10.5606/tgkdc.dergisi.2018.16078
- Dergi Adı: Turkish Journal of Thoracic and Cardiovascular Surgery
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.606-613
- Anahtar Kelimeler: Experimental, ischemia-reperfusion, lung transplantation, oxidative stress, proanthocyanidin
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Background: This study aims to investigate the preventive effect of proanthocyanidin against ischemia-reperfusion injury after lung transplantation. Methods: The study included 12 swines (weighing 35±5 kg) and separated into four groups. Groups 1 and 3 were identified as control groups and left upper lobectomy was performed. Groups 2 and 4 were identified as transplantation groups and left lower lobectomy and heterotransplantation were performed. Proanthocyanidin was only given to groups 3 and 4. Tissue samples were analyzed under light microscope and histopathological findings were recorded. Results: There was no statistically significant difference between control groups in terms of the numerical values of histopathological findings that include congestion (p=0.565), alveolar edema (p=0.197) and peribronchial inflammation (p=0.444). However, numerical values of acute cellular rejection were statistically significantly different between transplantation groups (p=0.048). Mean oxidative stress enzyme levels were higher in group 2 compared to group 4; however, the difference was not statistically significant (p > 0.05). Conclusion: According to the findings of our experimental study, proanthocyanidin can be safely used in lung transplantation based on its preventive effect in ischemia-reperfusion injury that may lead to morbidity and mortality. Amaç: Bu çalismada proantosiyanidinin akciğer nakli sonrasinda iskemi-reperfüzyon hasarini önleyici etkisi araştirildi. çalisma plani: çalişmaya 12 domuz (ağirlik, 35±5 kg) dahil edildi ve domuzlar dört gruba ayrildi. Grup 1 ve 3 kontrol gruplari olarak belirlendi ve sol üst lobektomi uygulandi. Grup 2 ve 4 nakil gruplari olarak belirlendi ve sol alt lobektomi ve heterotransplantasyon uygulandi. Proantosiyanidin sadece grup 3 ve 4'e verildi. Doku örnekleri işik mikroskopu altinda incelendi ve histopatolojik sonuçlar kaydedildi. Bulgular: Kontrol gruplari arasinda konjesyon (p=0.565), alveolar ödem (p=0.197) ve peribronşiyal enflamasyonu (p=0.444) içeren histopatolojik sonuçlarin sayisal degerleri açisindan istatistiksel olarak anlamli farklilik yoktu. Fakat akut hücresel reddin sayisal değerleri nakil gruplari arasinda istatistiksel olarak anlamli şekilde farkli idi (p=0.048). Ortalama oksidatif stres enzimi düzeyleri grup 2'de grup 4'e kiyasla daha yüksek idi, fakat farklilik istatistiksel olarak anlamli değildi (p > 0.05). Sonuç: Deneysel çalismamizin bulgularina göre, mobidite ve mortaliteye yol açabilen iskemi-reperfüzyon hasarini önleyici etkisine dayanarak proantosiyanidin akciger kanserinde güvenle kullanilabilir.