Rapid diagnosis of herpetic keratitis using direct immunoperoxidase technique


KALAYCI D., Eryilmaz T.

Acta Ophthalmologica, cilt.72, sa.1, ss.49-52, 1994 (Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 72 Sayı: 1
  • Basım Tarihi: 1994
  • Doi Numarası: 10.1111/j.1755-3768.1994.tb02736.x
  • Dergi Adı: Acta Ophthalmologica
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.49-52
  • Anahtar Kelimeler: direct immunoperoxidase, herpetic keratitis, immunoperoxidase, rapid diagnosis of herpetic keratitis
  • Sağlık Bilimleri Üniversitesi Adresli: Evet

Özet

Abstract. The direct immunoperoxidase technique was tested for diagnosis of herpetic keratitis. The technique was performed on corneal epitehlial specimens from 28 herpetic keratitis cases and 12 controls. Ulcers were stained with rose bengal or fluorescein and examined at the slit‐lamp biomicroscope to differentiate between herpetic and nonherpetic etiology. Scrapings obtained from the corneal epithelial ulcers were stained with direct immunoperoxidase technique. The whole procedure, including staining and examination of the slides, took a total of 70 min. The test proved to be accurate, easy to perform and to interpret, rapid and relatively inexpensive with a sensitivity of 93%, and a specificity of 100%. 1994 Institution Acta Ophthalmologica Scandinavica