The Potential Anti-inflammatory Effects of Naringenin on a Histamin Induced Otitis Media with Efussion Rat Model Histamin ile Oluşturulmuş Efüzyonlu Otitis Media Rat Modelinde Naringeninin Potansiyel Antiinflamatuar Etkileri

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İbas M., ARSLAN N., ÖÇAL R., Dursun S., Yumuşak N., KILIÇ R.

Journal of Ear Nose Throat and Head Neck Surgery, cilt.31, sa.4, ss.206-212, 2023 (Scopus, TRDizin)

Özet

Objective: The aim of this study was to histopathologically compare the effects of naringenin with methyl-prednisolone (MP) on a histamine induced otitis media with effusion (OME) animal model. Material and Methods: Twenty seven male Albino Wistar rats weigh- ing 250-300 g were used in this study. A histamine induced OME model was created by trans-tympanic 0.2 mL injections of 1 mg/mL histamine in both ears in all rats. Three groups of 9 rats were formed by a random selection. Group 1 did not receive any treatment, Group 2 re- ceived 1 mg/kg of intra-peritoneal MP treatment for 14 days and Group 3 received 50 mg/kg oral naringenin treatment. All rats were otomi- croscopically examined on the 48th hour in order to confirm the de- velopment of OME. At the end of the treatment, all rats were otomicroscopically re-examined and later sacrificed. The temporal bulla of all rats was examined with light microscopy and was evaluated in terms of submucosal neutrophil leucocyte count. Results: The first oto- microscopic evaluation revealed that 52 ears of 26 rats developed OME (96%). In the second oto-microscopic evaluation, OME was present in 16 ears (88%) in Group 1, 3 ears (19%) in Group 2 and 7 ears (39%) in Group 3. In terms of submucosal neutrophil count, a statistically sig- nificant difference was present between Group 1 and Group 2, and 3 (p<0.05) however no significant difference was present between Group 2 and 3 (p=0.21). Conclusion: Naringenin was found as effective as MP in both oto-microscopical examinations and histopathological eval- uations in a histamine induced animal model of OME.