Effects of Subcutaneous Bupivacaine and Morphine Infiltration on Mesenteric Ischemia–Reperfusion Injury in a Rat Model
Bratislava Medical Journal, cilt.127, sa.6, ss.2186-2198, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 127 Sayı: 6
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s44411-026-00590-6
- Dergi Adı: Bratislava Medical Journal
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Ultimate (EBSCO)
- Sayfa Sayıları: ss.2186-2198
- Anahtar Kelimeler: Ischemia, Bupivacaine, Morphine, OSI, Cortisol, Intestinal, Liver
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Background: Ischemia/reperfusion (I/R) injury is a clinical condition with high mortality, and an effective treatment strategy has yet to be established. Methods: This study was conducted on a total of 48 rats. They were divided into 6 groups of 8 rats each. Rats in Group S underwent laparotomy alone, while all other groups underwent laparotomy along with SMA I/R damage. Rats in Group B received 2 mg/kg/3 mL bupivacaine, rats in Group M received 0.5 mg/kg/3 mL morphine, rats in Group BM received 2 mg/kg/2 mL bupivacaine + 0.5 mg/kg/1 mL morphine, and rats in Group BML received 2 mg/kg/2 mL bupivacaine + 0.25 mg/kg/1 mL morphine subcutaneously. At the end of the study, blood samples were taken from all rats for oxidative stress parameters, ischemia markers, and cortisol measurement, and histopathological examination was performed. Results: Statistically significant differences were found among the groups with respect to mean total oxidant status (TOS), mean total antioxidant status (TAS), mean oxidative stress index (OSI), mean ischemia-modified albumin (IMA), and mean malondialdehyde (MDA) values (p < 0.001). Statistically significant differences were also observed among the groups in terms of mean corticosterone and cortisol levels (p < 0.001). Significant differences were found among the groups regarding mean intestinal histopathology score (IHS) and mean liver histopathology score (LHS) (respectively; p = 0.021, p = 0.002). Conclusions: The combination of morphine and bupivacaine provides effective analgesia and significantly improves oxidative stress parameters. The combined use of morphine and bupivacaine in infiltration anesthesia may be a promising treatment strategy for preventing I/R injury.