Therapeutic Effects of Carvedilol on Intestinal Mucosa in Intestinal Ischemia Reperfusion Injury Model; An Experimental Study
Journal of Indian Association of Pediatric Surgeons, cilt.31, sa.4, ss.630-637, 2026 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 31 Sayı: 4
- Basım Tarihi: 2026
- Doi Numarası: 10.4103/jiaps.jiaps_24_26
- Dergi Adı: Journal of Indian Association of Pediatric Surgeons
- Derginin Tarandığı İndeksler: Scopus, CINAHL, Directory of Open Access Journals, Academic Search Ultimate (EBSCO), Health Research Premium Collection (ProQuest)
- Sayfa Sayıları: ss.630-637
- Anahtar Kelimeler: Carvedilol, Chiu score, intestinal ischemia–reperfusion, oxidative stress, rat, thiol/disulfide homeostasis
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Background: Intestinal ischemia–reperfusion (I/R) injury is a critical clinical condition associated with high morbidity and mortality, with acute mesenteric ischemia carrying mortality rates of 60%–80%. Carvedilol, a third-generation β-blocker with known antioxidant properties, has demonstrated protective effects against I/R injury in various organs. This study aimed to investigate the effects of carvedilol on intestinal I/R injury in an experimental rat model, with assessment of oxidative stress parameters and histopathological damage. Materials and Methods: Thirty-two male Wistar albino rats were randomly divided into four groups (n = 8 each): sham, sham + carvedilol (2 mg/kg IP), I/R (30 min of superior mesenteric artery occlusion followed by reperfusion), and I/R + carvedilol (I/R plus 2 mg/kg carvedilol IP immediately after reperfusion). At 48 h postprocedure, blood samples were collected for biochemical analysis, including total antioxidant status (TAS), total oxidant status (TOS), and thiol/disulfide homeostasis parameters (sulfhydryl [SH], total thiol [TT], and disulfide bonds). Intestinal tissue samples were evaluated histopathologically using the Chiu scoring system. Results: Carvedilol significantly reduced intestinal mucosal damage, with the I/R + carvedilol group showing markedly lower Chiu scores compared to the untreated I/R group (median 2.0 vs. 4.0, P < 0.001). Biochemically, the I/R group exhibited significantly lower SH and TT levels than sham controls (P = 0.003 and P = 0.006, respectively), indicating oxidative stress. Carvedilol treatment preserved thiol status, with the I/R + carvedilol group demonstrating significantly higher SH levels than the untreated I/R group (P = 0.002). No significant differences were observed in TAS, TOS, or glutathione levels between the groups. Conclusion: Postreperfusion administration of carvedilol significantly attenuated intestinal I/R injury, as evidenced by reduced histopathological damage scores and preserved thiol/disulfide homeostasis. These findings suggest that carvedilol’s antioxidant properties may offer therapeutic potential in intestinal I/R injury. To our knowledge, this is the first study evaluating carvedilol in this context, warranting further investigation with different dosing regimens and timepoints.