Evaluation of Protective and Therapeutics Effects of Baicalein in Rat Kidney Stone Models Induced by Ethylene Glycol and Hydroxy-L-Proline
Journal of Urological Surgery, cilt.11, sa.4, ss.201-207, 2024 (ESCI, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 11 Sayı: 4
- Basım Tarihi: 2024
- Doi Numarası: 10.4274/jus.galenos.2024.2024-3-4
- Dergi Adı: Journal of Urological Surgery
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.201-207
- Anahtar Kelimeler: Baicalein, ethylene glycol, hydroxyproline, phytochemicals, rat kidney
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: To explore the effect of baicalein on the development and treatment of renal stones in a rat model induced by ethylene glycol or hydroxyproline. Materials and Methods: A grand total of 63 rats were split into nine distinct groups, each comprising seven rats: control, baicalein, ethylene glycol (EG), baicalein+ethylene glycol (BE), ethylene glycol+baicalein (EB), dimethyl sulfoxide (DMSO), hydroxy-L-proline (HP), baicalein+hydroxyproline (BH), and hydroxyproline+baicalein (HB) respectively. Urinary stone formation was induced in rats using EG or HP. Rat kidneys were examined by two histologists using light and electron microscopes. Calcium oxalate crystals and cellular changes were examined. Kidney injury molecule-1 (KIM-1), N-acetyl-β-D-glucosaminidase (NAG), neutrophil gelatinase-associated lipocalin (NGAL), osteopontin and interleukin-18 (IL-18) levels were measured as potential biomarkers in both the kidney tissue and blood. Results: There was no noticeable difference in histological features when the specimens were examined under a light microscope. Kidney stone formation and mitochondrial differences were observed in the EG and HP groups in electron microscopic (EM) examinations; however, EM findings were normal in all preventive and therapeutic groups. Serum IL-18 and KIM-1 levels were significantly lower in the therapeutic group than in the EG and HP groups (p<0.05). Conclusion: Baicalein has both protective and therapeutic roles in the management of kidney stone disease, and its therapeutic efficacy is superior to its protective efficacy. Further clinical studies in humans are needed to provide conclusive results on this issue.