Applications of MALDI-TOF MS in microbiological identification Mikrobiyolojik tanımlamada MALDI-TOF MS uygulamaları
TAF Preventive Medicine Bulletin, cilt.13, sa.5, ss.421-426, 2014 (Scopus, TRDizin)
- Yayın Türü: Makale / Derleme
- Cilt numarası: 13 Sayı: 5
- Basım Tarihi: 2014
- Doi Numarası: 10.5455/pmb.1-1371041898
- Dergi Adı: TAF Preventive Medicine Bulletin
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.421-426
- Anahtar Kelimeler: Bacterial identification, MALDI-TOF MS, Microbiological diagnostic
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
MALDI-TOF MS (Matriks assisted laser desorption ionization time of flight mass spectrometry) is a new metohod for identification of microorganisms nowadays. This method is based revealing of microorganisms protein profile with ionization of protein structure and these ionized mass pass through the electrical field. Profiles which were obtained from microorganisms compare with database of system thus identification is made by this way. Ribosomal proteins are used in identification which are less affected by enviromental conditions. Fresh culture should preferably use in MALDI-TOF MS identification. Ribosomal proteins can be deteriorate in old cultures. The correct identification rates are changing between 84,1% to 95,2% in routine bacterial isolates. The correct identification rates in yeasts are changing between 85% to 100%. It makes identification in positive blood culture bottles without the need of subculture, also makes identification on urine samples without the need of culture which has greater than 105 microorganisms in a microliter. When it compared with conventional and molecular identification methods, it is more effective on per sample costs and elapsed time on working.