Can Immature Granulocyte Counting be Utilized in the Selection of Samples for Flow Cytometric Enumeration of Stem Cells?
Clinical Laboratory, cilt.70, sa.10, ss.1914-1920, 2024 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 70 Sayı: 10
- Basım Tarihi: 2024
- Doi Numarası: 10.7754/clin.lab.2024.240345
- Dergi Adı: Clinical Laboratory
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1914-1920
- Anahtar Kelimeler: CD34, stem cell, immature granulocyte, flow cytome- try, hematology analyzer
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Background: This study aimed to compare the IMG counting by an auto hematology analyzer with the flow cytometric enumeration of CD34+ cells. Methods: All data from 124 samples submitted to the hematology laboratory for CD34+ cell counting in 2019 and 2020 were retrospectively evaluated. Whole blood samples were taken into EDTA tubes and assayed within 2 hours. The numbers and percentages of WBC and IMG were determined by using Mindray BC6200 hematology analyzer, while the same were determined for CD45 and CD34+ cells by using Beckman Coulter Navios flow cytometer. The performance of the new method was determined by the receiver operating characteristic (ROC) analysis. Results: Out of the 124 samples, 94 were collected from healthy individuals and 30 were collected from patients. A significant correlation was found between IMG and CD34+ cell counts in all patients (r = 0.71, p = 0.000) at the cutoff values of > 20/µL and > 50/µL. The Bland-Altman analysis of all patients showed that there was an agreement between the two methods. When the cutoff value of 20/µL for CD34+ cells was used in the ROC analysis, the sensitivity and specificity were calculated as 100 (96.1 - 100) and 96.77 (83.3 - 99.9), respectively, for the IMG count of > 900/µL. Conclusions: An IMG count of 900/µL can be used as the cutoff value in the analysis with the Mindray BC6200. The IMG counting cannot replace the flow cytometric CD34+ cell enumeration but can be used in the selection of the samples for stem cell enumeration by flow cytometry.