Encapsulation and Survival of a Chondrocyte Cell Line within Xanthan Gum Derivative
Macromolecular Bioscience, cilt.12, sa.3, ss.350-359, 2012 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 12 Sayı: 3
- Basım Tarihi: 2012
- Doi Numarası: 10.1002/mabi.201100304
- Dergi Adı: Macromolecular Bioscience
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.350-359
- Anahtar Kelimeler: cell delivery, microdroplet generator, microencapsulation, polysaccharides, xanthan gum
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
A chemical derivative of xanthan gum polysaccharide is investigated as a new artificial matrix for the encapsulation of chondrocytic cells. Toward this goal, a novel micro-droplet generator is developed to produce microcapsules. Microcapsules with an average diameter of 500μm, smooth surface, and homogeneous size distribution are obtained. ATDC5cells encapsulated in carboxymethyl xanthan (CMX) microcapsules remain viable and are observed to proliferate for prolonged culture periods with enhanced metabolic activity. Furthermore, retention of the chondrogenic phenotype is exhibited by the cells within CMX, suggesting the ability of this material to be applied in cell-delivery therapies. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.