Green synthesis of copper and zinc microflowers using Alkanna orientalis var. orientalis (L.) Boiss. and their potential biological activities
Chemical Papers, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s11696-026-05872-5
- Dergi Adı: Chemical Papers
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Chemical Abstracts Core, Materials Science & Engineering Collection (ProQuest), Technology Collection (ProQuest)
- Anahtar Kelimeler: <italic>Alkanna orientalis</italic>, Nanostructures, Bioinspired synthesis, Antimicrobial, Antioxidant, <italic>Leishmania tropica</italic>
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
In this study, copper and zinc hybrid microflowers (Cu-MFs and Zn-MFs) were synthesized using Alkanna orientalis var. orientalis extract (AOE) as a bioactive organic component and comprehensively evaluated for their structural and biological properties. FE-SEM, EDX, XRD, FTIR, DLS, and zeta potential analyses confirmed the synthesized MFs’ hierarchical flower-like morphology and metal phosphate-based inorganic phases. HPLC–DAD analysis of AOE identified several phenolic acids and flavonoids, with rosmarinic acid being the major quantified compound. Cu-MFs exhibited stronger radical-scavenging activity than AOE, with IC50 values of 112.8 µg/mL for DPPH and 58.4 µg/mL for ABTS, whereas Zn-MFs showed IC50 values of 318.4 and 160.2 µg/mL, respectively. Both Cu-MFs and Zn-MFs also showed improved antimicrobial activity compared with AOE and the corresponding phosphate controls. In the antileishmanial assays, AOE was more active against extracellular promastigotes, while Zn-MFs showed a favorable activity profile against intracellular amastigotes, with an IC50 of 216.19 µg/mL in the Trypan Blue assay and 1361 µg/mL in the macrophage IC50 assay. Cu-MFs exhibited the highest peroxidase-like catalytic activity among the tested materials. Overall, these findings demonstrate the multifunctional potential of A. orientalis-mediated Cu- and Zn-MFs and provide a basis for further investigation of their structural, catalytic, and biological properties. This study’s innovation lies in the green synthesis of copper and zinc hybrid MFs using AOE and the comprehensive evaluation of their structural, antioxidant, catalytic, antimicrobial, and antileishmanial properties in a single integrated study.