Investigation of Myelin Basic Protein Activity in Determining Neuronal Damage in Rats with Experimental Head Trauma Deneysel Kafa Travması Oluşturulan Sıçanlarda Nöronal Hasarı Belirlemede Myelin Basic Protein Etkinliğinin Araştırılması

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ÇELEBİ H., ASLAN Ş., DURAK V. A., AYDİN B., ÇIKRIKLAR H. İ.

Anatolian journal of emergency medicine, cilt.6, sa.2, ss.61-65, 2023 (Scopus, TRDizin)

Özet

Aim: In our study, mild traumatic brain injury was performed in rats after experimentally induced head trauma. Our aim was to investigate the diagnostic value of myelin basic protein (MBP) level in serum samples taken in the acute period of traumatic brain injury. Material and Methods: A total of 40 adult Spraque-Dawley rats were used in our study. The subjects were divided into 5 groups as the control group (n=8) and 4 different experimental groups (n=8). The model described by Marmarou et al. was modified and applied for the trauma. In the model that we designed, the balls with different weights and heights were released by the free fall method. It was aimed to create trauma with a severity of 0.05, 0.1, 0.2, 0.4 Newtons and the groups were named A, B, C, and D. MBP levels were evaluated in blood samples taken from rats 2 hours after trauma. Results: Compared with the control group, blood MBP levels were found to be significantly higher in groups A and B. When the groups are compared among themselves, there was a significant difference between the B and C groups. Similarly, a significant difference was found between groups A and C and groups A and D. When the four groups were compared among themselves, no correlation was found between the severity of trauma and the blood level of MBP. Conclusion: The fact that the values are different in the groups with different trauma severity strengthens the possibility that the increase in MBP is also due to secondary causes. MBP may be a valuable biomarker to evaluate TBI in head trauma, but more studies should be carried out on this field.