Hippo signaling pathway in human testis and seminoma: anticancer effect of verteporfin on human seminoma TCam-2 cells
Journal of Molecular Histology, cilt.56, sa.5, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 56 Sayı: 5
- Basım Tarihi: 2025
- Doi Numarası: 10.1007/s10735-025-10565-6
- Dergi Adı: Journal of Molecular Histology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, EMBASE, MEDLINE
- Anahtar Kelimeler: Human testis, Seminoma, TCam-2, Hippo, Verteporfin
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Aim: Seminoma and embryonal carcinoma are among the most common germ cell tumors. Verteporfin, a YAP-TEAD inhibitor, has emerged as a potential anticancer agent. This study investigated the localization of Hippo signaling proteins in human testis, seminoma, and non-seminoma tissues, and examined verteporfin’s effects on TCam-2 seminoma cells. Methods: Protein localization in tissues was assessed using immunohistochemistry. TCam-2 cells were treated with various concentrations of verteporfin (1–40 µM) for 24, 48, and 72 h. Protein and gene expression were analyzed via immunofluorescence, western blotting, and qRT-PCR. Results: Hippo pathway proteins showed distinct localization patterns across tissue types. Verteporfin treatment caused a dose- and time-dependent decrease in protein levels without altering mRNA expression. It also induced nuclear-to-cytoplasmic translocation of pathway proteins and reduced cell proliferation, migration, and viability, while simultaneously promoting apoptosis in TCam-2 human seminoma cells. Conclusion: Verteporfin may serve as a promising therapeutic strategy for seminoma by targeting Hippo signaling. These findings support its potential role in personalized treatment approaches for testicular cancer.