Protective effects of montelukast on ischemia-reperfusion injury in rat ovaries subjected to torsion and detorsion: Biochemical and histopathologic evaluation
Fertility and Sterility, cilt.95, sa.4, ss.1360-1366, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 95 Sayı: 4
- Basım Tarihi: 2011
- Doi Numarası: 10.1016/j.fertnstert.2010.08.017
- Dergi Adı: Fertility and Sterility
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1360-1366
- Anahtar Kelimeler: Histopathology, ischemia, montelukast, ovary, oxidative stress, rat, reperfusion
- Sağlık Bilimleri Üniversitesi Adresli: Hayır
Özet
Objective: To reveal the effects of montelukast as an antioxidant and tissue protective agent and study the biochemical and histopathologic changes in experimental ischemia and ischemia-reperfusion (I/R) injury in rat ovaries. Design: Experimental study. Setting: Experimental surgery laboratory in a university department. Animal(s): Forty-eight rats with experimentally induced ovarian torsion. Intervention(s): Group 1: sham; Group 2: ovarian ischemia; Group 3: a 30-hour period of ischemia followed by a 3-hour reperfusion. Groups 4 and 5: rats administered 10 and 20 mg/kg doses of montelukast before a half-hour of ischemia, then ovarian ischemia applied; after a 3-hour period of ischemia, the bilateral ovaries removed. Groups 6 and 7: 3-hour period of ovarian ischemia applied, then 2.5 hours after the ischemia induction, rats given montelukast. Group 8: sham operation and 20 mg/kg of montelukast; at the end of a 3-hour period of ischemia, 3-hours of reperfusion continued. Main Outcome Measure(s): Measurement of ovarian tissue concentrations of superoxide dismutase (SOD), glutathione (GSH), lipid peroxidation (LPO) and myeloperoxidase (MPO) activity; and histopathologic examination of all ovarian rat tissue. Result(s): Montelukast treatment normalized changes of LPO and MPO and stimulated an overproduction of endogenous SOD and GSH. The results of the histologic parameters showed that treatment with montelukast in the I/R group of rats ameliorated the development of ischemia and reperfusion tissue injury. Conclusion(s): Montelukast at different doses attenuates ovarian I/R-induced ovary tissue injury in rats. © 2011 American Society for Reproductive Medicine, Published by Elsevier Inc.