The effects of chlorhexidine-based preservative tubes on routine urinalysis stability
International Journal of Medical Biochemistry, cilt.3, sa.3, ss.155-160, 2020 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 3 Sayı: 3
- Basım Tarihi: 2020
- Doi Numarası: 10.14744/ijmb.2020.52824
- Dergi Adı: International Journal of Medical Biochemistry
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.155-160
- Anahtar Kelimeler: Chlorhexidine, preanalytical phase, storage, urinalysis, urine
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objectives: To evaluate the effects of chlorhexidine-based preservative tubes on analytes for routine urinalysis. Methods: Urine specimen (n=84) aliquots in polystyrene tubes (PS) with no additives (Fıratmed Co. Ltd., Istanbul, Turkey) and in Becton–Dickinson Vacutainer® Plus Urinalysis Preservative (BD-UAP) tubes (Becton Dickinson Inc., NJ, USA) were analyzed on automatic modular urine analyzer (Dirui Industry, Changchun, China) and the results were com-pared. Stability was assessed by re-analyzing urine samples in BD-UAP tubes after three and six hours of storage and comparing with initial results. Cohen’s kappa coefficient (κ) was calculated to assess the agreement of results. Results: Squamous epithelial cell and pH agreement between the tubes was moderate (κ=0.60 and 0.58, respectively). Bilirubin, ketone, protein, nitrite, and glucose showed perfect agreement with the PS tube and remained stable for up to six hours in the BD-UAP tube (κ=1.00, 0.92, 0.83, 0.93, 1.00, respectively). Red blood cells, white blood cells, bacte-ria, blood, and leukocyte esterase showed moderate to substantial stability at six hours (κ=0.56, 0.77, 0.76, 0.87, 0.57, respectively). Conclusion: Although storage in chlorhexidine-based preservative tubes has limited effects on chemical strip analysis, these tubes may not be considered optimal for microscopic examination.