Endothelial cell viability of donor corneas preserved in Eusol-C corneal storage medium
Experimental and Clinical Transplantation, cilt.14, sa.4, ss.441-444, 2016 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 14 Sayı: 4
- Basım Tarihi: 2016
- Doi Numarası: 10.6002/ect.2014.0295
- Dergi Adı: Experimental and Clinical Transplantation
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.441-444
- Anahtar Kelimeler: Eusol-C, Corneal storage medium, Endothelial cell density
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objectives: To evaluate the efficacy of Eusol-C as a corneal storage medium on the survival of donor endothelium. Materials and Methods: Twenty-seven corneas not suitable for transplant were included in this study. All donor corneas were stored in Eusol-C at 4°C. Daily donor corneal endothelial cell counting was performed with an eye bank specular microscope. All corneas were discarded after the study process. Results: Mean donor age was 51.3 ± 18.8 years (range, 25-94 y). The mean duration between death and corneal excision was 9.5 ± 6.7 hours (range, 3-23 h). Mean endothelial cell density was 2195 ± 383 cells/mm2 at the beginning of the preservation (range, 1361-2899 cells/mm2). Donor endothelial cell density was between 1500 to 2000 cells/mm2 in 9 corneas, 2000 to 2500 in 11 corneas, 2500 to 3000 in 5, and higher than 3000 in 2 corneas at baseline. Mean endothelial cell density was found 1658 cells/mm2 on the eighth day of storage, with a mean endothelial cell loss rate of 24.5%. Corneas stored 9 to 24 days in Eusol-C had a rate of endothelial cell damage of 3.1% per day. Conclusions: Although our results revealed a higher endothelial cell loss than previous reports, overall performance of Eusol-C in preserving the donor endothelium may be satisfactory for clinical use.