Coupling dalapon biodegradation with electricity generation in microbial fuel cells

Sarioglu Y., SEVER KAYA D., KURT H., Kantarci-Carsibasi N., KUL A., MARAKLI S., ...Daha Fazla

Bioresource Technology, cilt.445, 2026 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 445
  • Basım Tarihi: 2026
  • Doi Numarası: 10.1016/j.biortech.2026.134073
  • Dergi Adı: Bioresource Technology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, Compendex, EMBASE, Environment Index, Geobase, INSPEC, MEDLINE, Academic Search Ultimate (EBSCO), Engineering Source (EBSCO)
  • Anahtar Kelimeler: Microbial biotechnology, Microbial community, Pesticides, Molecular docking, Molecular Dynamics
  • Sağlık Bilimleri Üniversitesi Adresli: Evet

Özet

Dalapon (2,2-dichloropropionic acid) is a persistent halogenated herbicide frequently detected in aquatic environments, yet its bioelectrochemical degradation has not been previously demonstrated. This study reports the simultaneous biodegradation of dalapon and electricity generation in single-chamber microbial fuel cells inoculated with the psychrotolerant Antarctic isolate Psychrobacter sp. TaeBurcu001. While mixed microbial cultures alone were unable to oxidize dalapon as the sole added carbon source, co-inoculation with TaeBurcu001 enabled measurable electricity generation (0.1–0.21 V at 980 Ω) and achieved more than 90% dalapon removal. Targeted LC–MS/MS analysis confirmed substantial dalapon degradation under all tested conditions. Microbial community analysis based on 16S rRNA gene sequencing revealed enrichment of electrogenic and xenobiotic-degrading genera, including Xanthobacter, Pseudomonas, Achromobacter, and Dysgonomonas. Molecular docking and molecular dynamics simulations suggested favorable binding of dalapon within the catalytic pocket of L-2-haloacid dehalogenase, supporting a plausible enzymatic contribution to dehalogenation. Overall, this study demonstrates the potential of using specialized pollutant-degrading bacteria to enhance the functionality of MFCs for treating recalcitrant organic contaminants.