The effect of selenium on amikacin-induced ototoxicity
Journal of International Advanced Otology, cilt.9, sa.2, ss.219-226, 2013 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 9 Sayı: 2
- Basım Tarihi: 2013
- Dergi Adı: Journal of International Advanced Otology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.219-226
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Purpose: The aim of this study was to investigate the possible protective effects of selenium on amikacin-induced ototoxicity in rats. Materials and Method: In this study, 32 healthy Wistar albino rats were used as test animals. After measurements of distortionproduct otoacoustic emissions (DPOAE), the rats were divided into four groups. The rats in the "control" group were injected with intramuscular 1 ml/kg/day 0.9% NaCI (saline) for 28 days. The rats in the "amikacin" group received intramuscular 200 mg/kg/day amikacin for 28 days. The rats in the "Amikacin+Selen60" group received intramuscular 200 mg/kg/day amikacin and intraperitoneal low dose of selenium (60 μg/kg/day) for 28 days. The rats in the "Amikacin+selenium 300" group received intramuscular 200 mg/kg/day amikacin and intraperitoneal high dose of selenium (300 μg/kg/day) for 28 days. The experimental groups were evaluated with DPOAE at day 1, day 14 and day 28. Results: In the "amikacin" group, there were significant hearing loss in DPOAE audiogram (DPgram) measurements at the days 14 and day 28 compared to day 1 (p < 0.01). In both "amikacin+selenium60" (group 3) and "amikacin+selenium300" (group 4), there were significant hearing loss in day 14 compared to day 1 (p< 0.01); there were also significant hearing loss in day 28 compared with day 14 (p< 0.01). Differences in ototoxicity in the Dpgrams were assessed between the groups (repeated measures ANOVA and post-hoc Tukey test); there were no significant difference in all frequencies between "amikacin" group, "amikacin+selenium60" group and "amikacin+selenium300" group (p > 0.05). Conclusion: A statistically significant hearing loss was detected in all groups. The long duration of selenium treatment were assessed in rats and both the time and the dose of selenium had no the protective effects on amikacin-induced ototoxicity.