Comparative relaxant effects of YC-1 and DEA/NO on the sheep sphincter of Oddi
Pancreatology, cilt.6, sa.3, ss.215-219, 2006 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 6 Sayı: 3
- Basım Tarihi: 2006
- Doi Numarası: 10.1159/000091959
- Dergi Adı: Pancreatology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.215-219
- Anahtar Kelimeler: sphincter of Oddi, relaxation, YC-1, DEA/NO
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Background/Aims: Nitric oxide (NO) is a major inhibitor in various parts of the gastrointestinal tract. This study was designed to compare the effects of YC-1, NO-independent soluble guanylate cyclase (sGC) activator, and DEA/NO, NO-nucleophile adduct, on sheep sphincters of Oddi (SO). Methods: SO rings were mounted in a tissue bath and tested for changes in isometric tension in response to 3-(5′-hydroxymethyl-2′-furyl)-1-benzyl indazole (YC-1, 10- 10-10-5 M), diethylamine/NO complex (DEA/NO, 10 -8-10-4 M). We also evaluated the effect of YC-1 (10 -6 and 10-5 M) and DEA/NO (10-5 and 10 -4 M) on the levels cyclic GMP (cGMP) in isolated SO. Results: YC-1 (10-10-10-5 M) and DEA/NO (10-8-10-4 M) induced concentration-dependent relaxation of isolated SO rings precontracted with carbachol (10-6 M). The pEC50 value of DEA/NO was significantly lower than those for YC-1 (p < 0.05), with no change of Emax values. YC-1 increased cGMP levels more than control, carbachol and DEA/NO groups (p < 0.05). Conclusion: These results show that YC-1 is a more potent relaxant than DEA/NO and causes more elevation of cGMP levels in isolated SO rings. Copyright © 2006 S. Karger AG and IAP.