Use of alkaline Comet assay (single cell gel electrophoresis technique) to detect DNA damages in lymphocytes of operating room personnel occupationally exposed to anaesthetic gases

Şardaş, S.; Aygün, N.; GAMLI, Mehmet; ÜNAL, YUSUF; Ünal, N.; Berk, N.; Karakaya, A.E.

doi:10.1016/s1383-5718(98)00113-2

Use of alkaline Comet assay (single cell gel electrophoresis technique) to detect DNA damages in lymphocytes of operating room personnel occupationally exposed to anaesthetic gases

Şardaş S., Aygün N., GAMLI M., ÜNAL Y., Ünal N., Berk N., ...Daha Fazla

Mutation Research - Genetic Toxicology and Environmental Mutagenesis, cilt.418, sa.2-3, ss.93-100, 1998 (Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 418 Sayı: 2-3
Basım Tarihi: 1998
Doi Numarası: 10.1016/s1383-5718(98)00113-2
Dergi Adı: Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Derginin Tarandığı İndeksler: Scopus
Sayfa Sayıları: ss.93-100
Anahtar Kelimeler: comet assay, single cell gel electrophoresis, DNA single strand break, DNA damage, anaesthetic gas
Sağlık Bilimleri Üniversitesi Adresli: Evet

Özet

Here, we report the possible in vivo induction DNA damage by exposure to various waste anaesthetic gases such as halothane, nitrous oxide and isoflurane. The alkaline comet assay (single cell gel electrophoresis technique) was carried out on 66 operating room personnel (anaesthetists [doctors]; anaesthesia nurses and anaesthesia unit technicians) currently employed at the Ankara Hospital in Turkey. A significant increase in the number of lymphocytes with DNA migration was observed in operating room personnel as compared to controls. Also, the extent of damage in exposed smokers were significantly higher than exposed nonsmokers. This study supports the existence of an association between DNA damage and occupational exposure to inhalation anaesthetics. Copyright (C) 1998 Elsevier Science B.V.