Enzymatic degradation behaviour of starch conjugated phoshorylated chitosan
3rd International Materials Symposium and 12th Portuguese Materials Society Meeting 2005, Aveiro, Portekiz, 20 - 23 Mart 2005, cilt.514-516, ss.995-999, (Tam Metin Bildiri)
- Yayın Türü: Bildiri / Tam Metin Bildiri
- Cilt numarası: 514-516
- Doi Numarası: 10.4028/www.scientific.net/msf.514-516.995
- Basıldığı Şehir: Aveiro
- Basıldığı Ülke: Portekiz
- Sayfa Sayıları: ss.995-999
- Anahtar Kelimeler: chitosan, starch, enzymatic degradation, biomaterials
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Phosphorylated chitosan (P-chitosan) was synthesized by means of graft copolymerization technique. The conjugate membranes were prepared from oxidised starch and Pchitosan using reductive alkylation crosslinking. The synthesized membranes were characterised by FT-IR. In order to characterize degradation behaviour of this conjugated system, the membranes were incubated in enzyme solutions of alpha-amylase and lysozyme as well as a physiological saline solution (PBS) used as control solution. In PBS, low starch containing membranes (0.16-0.38 weight (starch)/weight (P-chitosan), (w s/wc)) and control membranes have not showed significant change in their weight during two months of incubation. High starch containing membranes (0.73-1.04 ws/wc) indicated less than 20 % weight loss after this period. After α-amylase incubation, a distinct degradation behaviour was observed from starch-P-chitosan membranes. The degradation of the conjugate membranes was found to be fast with increasing starch content. Weight losses between 20 to 55 % were detected for the lowest (0.16 ws/wc) starch and highest (1.04 ws/w c) starch containing membranes, respectively. In the lysozyme degradation study, the conjugate membranes were not degraded by enzymatic activity and the weights of membranes were seen to be increased about 20 % because of swelling. The control membranes showed gradual weight loss in enzyme solutions. These results indicated the lysozyme degradation of starch-free P-chitosan membranes and inhibition of degradation P-chitosan by highly conjugated starch molecules. chitosan, starch, enzymatic degradation, biomaterials.