Does metformin protect against brain injury after subarachnoid hemorrhage? An experimental study Subaraknoid kanama sonrası metformin erken beyin hasarını önleyebilir mi? Deneysel çalışma
Turkiye Klinikleri Journal of Medical Sciences, cilt.40, sa.4, ss.393-398, 2020 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 40 Sayı: 4
- Basım Tarihi: 2020
- Doi Numarası: 10.5336/medsci.2020-76488
- Dergi Adı: Turkiye Klinikleri Journal of Medical Sciences
- Derginin Tarandığı İndeksler: Scopus, CAB Abstracts, CINAHL, EMBASE, Veterinary Science Database, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.393-398
- Anahtar Kelimeler: Early brain injury, Hyperglycemia, Metformin, Subarachnoid hemorrhage
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: Hyperglycemia is one of the main risk factors for early brain injury and an outcome in patients with subarachnoid hemorrhage (SAH). Agents that regulate blood glucose levels can prevent the development of early brain injury. The present experimental study aimed to investigate the histological and anti-apoptotic effects of met-formin on SAH in a rat model. Material and Methods: An SAH model was applied in 28 male Wistar rats who were randomly grouped as fol-lows: control (Group 1), SAH alone (Group 2), SAH with hyperglyce-mia (Group 3), and metformin-treated SAH with hyperglycemia (Group 4). Intraperitoneal streptozotocin (50 mg/kg) was administered for 7 d to induce hyperglycemia before conducting the experiment. All rats were sacrified 72 hours after SAH. Histopathological and immunohistoche-mical evaluations were also conducted. Results: The thickness of the basilar artery wall was significantly different among the four groups (p=0.003). The thickness of the basilar artery wall was higher in Group 3 than in Groups 1 and 2. Blood glucose values were significantly different among the four groups (p<0.001). The number of neurons (glial cells) stained with terminal deoxynucleotidyl transferase dUTP nick end labeling was lower in Group 4 than in Group 3 (p=0.025). Conclusion: The main findings of our study indicate that metformin regulates blood glucose levels in hyperglycemic rats with SAH. Our results suggest that metformin may protect neurons against apoptosis.