An experimental study on the effects of splenectomy on bone fracture healing
Signa Vitae, cilt.19, sa.6, ss.86-94, 2023 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 19 Sayı: 6
- Basım Tarihi: 2023
- Doi Numarası: 10.22514/sv.2023.067
- Dergi Adı: Signa Vitae
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, Central & Eastern European Academic Source (CEEAS), Veterinary Science Database
- Sayfa Sayıları: ss.86-94
- Anahtar Kelimeler: Splenectomy, Fracture healing, Experimental rat study
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Bone fracture healing is a complex process involving multiple mechanisms where immune system has vital role. The spleen is a part of this process and its effect on bone metabolism is not completely understood. Herein, the effects of simultaneous bone fractures and splenectomy were evaluated regarding early and late periods of fracture healing in rats. The closed fracture model was applied in sixty-four healthy male Wistar-albino rats. Rats were randomly selected and divided into four groups. The splenectomy was carried out in two groups. The early period was declared as three weeks and late period as five weeks for evaluating the fracture healing in rats, (Groups A: splenectomized 3rd week, Groups B: non-splenectomized 3rd week, Groups C: splenectomized 5th week, and Groups D: non-splenectomized 5th week). The groups were compared regarding radiological, biomechanical, and histopathological aspects at 3rd and 5th weeks of fracture healing. The callus/bone volume ratios of Groups C and D were lower than those of Groups A and B (A–C, p ≤ 0.001; B–D, p ≤ 0.001). Radiographic Union Score for Tibial Fracture scores of Groups C and D were higher than those for Groups A and B (A–C, p ≤ 0.001; B–D, p ≤ 0.001). Bone mineral density measurements of Groups C and D were higher than those for Groups A and B (A–C, p ≤ 0.001; A–D, p ≤ 0.001; B–C, p ≤ 0.001 and B–D, p ≤ 0.001). The histological scoring of Groups A was lower than Groups C and D (A–C, p = 0.004; A–D, p = 0.001). Splenectomy may thus adversely affect the early phase fracture healing.