Phytochemical analysis and antioxidant, antimicrobial, cytotoxic activities of different solvent extracts of Zygophyllum fabago L. Análise fitoquímica e atividades antioxidante, antimicrobiana e citotóxica de diferentes extratos solventes de Zygophyllum fabago L.

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Mamurova A., Kairanova G., BEYATLI A., Smagulova G., Yedilova A., Zhaldybayev K., ...Daha Fazla

Brazilian Journal of Biology, cilt.85, 2025 (Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 85
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1590/1519-6984.293666
  • Dergi Adı: Brazilian Journal of Biology
  • Derginin Tarandığı İndeksler: Scopus, MEDLINE, Directory of Open Access Journals
  • Anahtar Kelimeler: biological activities, DPPH, IC50, MIC, phytochemicals, Z. fabago
  • Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
  • Sağlık Bilimleri Üniversitesi Adresli: Evet

Özet

This study investigates the phytochemical composition, antioxidant, antimicrobial, and cytotoxic properties of six solvent extracts (ethanol, ethyl acetate, and hexane) obtained from the roots and aerial parts of Zygophyllum fabago L. growing in the arid and saline regions of southern Kazakhstan. Antioxidant potential was assessed using DPPH and ABTS radical scavenging assays, with Trolox as a reference standard. The minimum inhibitory concentration (MIC) of the extracts was determined using the broth microdilution method. The ethanol extract of aerial parts demonstrated the highest total phenolic content (128.29 ± 0.81 mg GAE/g) and the strongest antioxidant activity (DPPH SC50 = 42.57 ± 3.03 µg/mL), where SC50 represents the concentration required to scavenge 50% of free radicals. Ethyl acetate extracts exhibited significant antimicrobial activity, particularly against E.coli and C.albicans (MIC <9 µg/mL). Cytotoxicity was assessed using the MTT assay on NIH-3T3, K562, and Saos cell lines. The ethyl acetate extracts (both roots and aerial parts) were most effective against K562 leukemia cells, with IC50 values of 121.0 ± 2.5 µg/mL and 120.4 ± 3.1 µg/mL, respectively. These findings highlight the potential of Z. fabago extracts as natural antioxidants and antimicrobial agents.