Caffeic acid phenethyl ester alleviates mesenteric ischemia/reperfusion injury
Journal of Investigative Surgery, cilt.25, sa.6, ss.354-365, 2012 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 25 Sayı: 6
- Basım Tarihi: 2012
- Doi Numarası: 10.3109/08941939.2012.677968
- Dergi Adı: Journal of Investigative Surgery
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.354-365
- Anahtar Kelimeler: caffeic acid phenethyl ester, ischemia/reperfusion, mesenteric ischemia, reperfusion injury, oxidative stress, intestinal mucosal injury
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Purpose: We aimed to investigate the effects of caffeic acid phenethyl ester (CAPE) on intestinal mucosal injury induced by superior mesenteric occlusion. Methods: This experimental study was conducted on 48 male Wistar-Albino rats. The animals were randomly allocated into four groups: (i) Sham-operated group, laparotomy without intestinal ischemia/reperfusion (IR) injury (n12); (ii) Sham CAPE group, identical to group 1 except for CAPE treatment (10 μmol/kg, intravenously) (n12); (iii) Intestinal IR group, 60 min of superior mesenteric ischemia followed by 3 hr of reperfusion (n12); and (iv) (IR CAPE)-treated group, 10 μmol/kg injection of CAPE intravenously 30 min before the reperfusion period (n12). We evaluated the degree of intestinal mucosal injury on a grading scale, histopathologically, and by measuring oxidative stress markers and antioxidant parameters, biochemically. Intestinal edema was estimated by using wet/dry weight ratios. The plasma proinflammatory cytokine levels were measured. Animal survival was observed up to one week. Results: Intestinal mucosal injury scores were significantly decreased with CAPE administration (p < .05). CAPE treatment significantly reduced oxidative stress markers in the intestinal tissues (p < .05) and the plasma proinflammatory cytokine levels (p < .05), and significantly increased antioxidant parameters in the intestinal tissues (p < .05). Intestinal edema was significantly alleviated by CAPE treatment (p < .05). The survival rates of CAPE-treated IR animals were significantly higher than IR-subjected rats (p < .05). Conclusion: This study clearly showed that CAPE treatment significantly alleviated the intestinal mucosal injury caused by superior mesenteric ischemia/reperfusion. Further clinical studies are required to clarify whether CAPE has a useful role in reperfusion injury during particular surgeries in which IR-induced organ injury occurs. © 2012 Informa Healthcare USA, Inc.