Determination of carvedilol in human plasma by gas chromatography-mass spectrometry method
Journal of Chromatographic Science, cilt.49, sa.1, ss.35-39, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 49 Sayı: 1
- Basım Tarihi: 2011
- Doi Numarası: 10.1093/chrsci/49.1.35
- Dergi Adı: Journal of Chromatographic Science
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.35-39
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
An analytical procedure was developed and validated for the quantitation of carvedilol in human plasma. Carvedilol and atenolol (internal standard) were extracted from human plasma with a mixture of diethylether and ethylacetate at basic pH with liquid-liquid extraction. The extracts were derivatized with n-Methyl-n-(trimethylsilyl)trifluoroacetamide and analyzed by gas chromatography-mass spectrometry (GC-MS). Calibration curves were linear over the concentration range 15-500 ng/mL Intra-and inter-day precision, expressed as the relative standard deviation were less than 8.0%, and accuracy (relative error) was better than 11.0%. The limits of detection and quantification of carvedilol were 5.0 and 15 ng/mL, respectively. Also, the method was successfully applied to three patients with hypertension who had been given an oral tablet of 25 mg carvedilol.