EFFECT OF MESENCHYMAL STEM CELL AND ERYTHROPOIETIN COMBINATION IN A RAT SPINAL FUSION MODEL
Journal of Turkish Spinal Surgery, cilt.34, sa.1, ss.36-44, 2023 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 34 Sayı: 1
- Basım Tarihi: 2023
- Doi Numarası: 10.4274/jtss.galenos.2023.40085
- Dergi Adı: Journal of Turkish Spinal Surgery
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.36-44
- Anahtar Kelimeler: erythropoietin, MDCT, mesenchymal stem cell, Mimics Innovation Suite, spinal fusion, Sprague-Dawley rat
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: The benefits of erythropoietin (EPO) or mesenchymal stem cell (MSC) application during spinal fusion were discussed in the literature. Still, the effect of the combination may enhance favorable outcomes. This study compared the efficacy of MSCs and EPO treatments separately and together in a rat model. Materials and Methods: This study was designed as an experimental, controlled animal study. The groups consisted of EPO or MSC application or both: PreS - EPO + MSC (EPO starting 24 h before the surgery) or PostS - EPO + MSC (EPO starting 72 h after the surgery) with control groups. Experimental posterolateral L4-L5 spinal fusion was performed. Plain radiographs and multi-detector computed tomography scans were performed for the rats preoperatively and at the 3rd and 6th weeks. Using the Mimics Innovation Suite, 3D models of the fusion site were reconstructed, volume analysis and volumetric changes in these periods were calculated. Manuel palpation assessment and histopathological analyses were also performed to assess the fusion. Results: Radiologically, the fusion rate at weeks 3 and 6 were significantly higher in the EPO + MSC groups than that in the EPO group alone. The highest bone-volume increase was detected in the PostS - EPO + MSC groups. The PreS - EPO + MSC-3 group and the PostS - EPO + MSC-6 group had the highest fusion rates according to manual palpation (p=0.048, 71.4%). The EPO groups had lower fusion rates compared to those in the control and MSC groups (14.3 % both at the 3rd and 6th weeks). The PreS - EPO + MSC-3 group had the highest histological score among the groups. The EPO-6 and PostS - EPO + MSC-6 groups had the lowest scores with respect to histological examination. Conclusion: The combination of EPO + MSC application showed additionally significant benefits according to radiological and histological examination but EPO adversely affected the fusion.