The role of miR-145, miR-200c, and miR-21 in distinguishing between hyperplastic and adenomatous colon polyps and colorectal cancer prediction
Croatian Medical Journal, cilt.66, sa.5, ss.345-351, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 66 Sayı: 5
- Basım Tarihi: 2025
- Doi Numarası: 10.3325/cmj.2025.66.345
- Dergi Adı: Croatian Medical Journal
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Central & Eastern European Academic Source (CEEAS), EMBASE, MEDLINE, Directory of Open Access Journals
- Sayfa Sayıları: ss.345-351
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Aim To assess whether miRNA expression can be a biomarker for distinguishing between hyperplastic and adenomatous polyps and colon cancer tissues. Methods The study involved 40 colon adenocarcinoma (CA) tissue samples, 40 low-grade adenomatous (A) polyps, and 40 hyperplastic (HP) polyps. The samples were formalin-fixed and paraffin-embedded. Quantitative real-time polymerase chain reactions were used to determine the expression levels of miR-21, miR-200c, and miR-145. Results miR-145 expression was significantly lower in the CA than in both the A and HP groups (P=0.012 and P=0.004, respectively) and in the A than in the HP group (P=0.028). miR-200c expression was higher in the CA than in the A (P=0.008) and HP groups (P=0.009), while no difference was observed between the A and HP groups (P=0.624). miR-21 showed a stepwise increase across the groups, with the highest levels in the CA, followed by the A and HP groups (P<0.001 for both CA vs A and CA vs HP; P=0.037 for A vs HP). Conclusion The study revealed dysregulated expression of miR-145 and miR-21 in colon polyps and cancer tissue, showing that these miRNAs may be used to distinguish between hyperplastic and adenomatous polyps and predict colorectal cancer.