Comparison of the efficacy of melatonin and 1400w on renal ischemiareperfusion injury: A role for inhibiting iNOS
Renal Failure, cilt.31, sa.8, ss.704-710, 2009 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 31 Sayı: 8
- Basım Tarihi: 2009
- Doi Numarası: 10.3109/08860220903085989
- Dergi Adı: Renal Failure
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.704-710
- Anahtar Kelimeler: melatonin, 1400W, renal ischemia/reperfusion, oxidative stress, peroxynitrite
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Introduction. We investigated the roles of melatonin (a powerful antioxidant, iNOS inhibitor, and a scavenger of peroxynitrite) and 1400W (a strong and selective inhibitor of inducible nitric oxide) on renal dysfunction and injury induced by ischemiareperfusion (IR) of rat kidney, since oxidative and nitrosative injury are believed to be the major causes. Materials and methods. Thirty-two male Sprague-Dawley rats were divided into four groups of sham-operated, IR, IR Melatonin and IR 1400W. Rats were given either melatonin (10 mgkg) or 1400W (10 mgkg) in the IR Melatonin and IR 1400W groups respectively at 6 h prior to ischemia and at the beginning of reperfusion via intraperitoneal route. IR injury was induced by 60 min of bilateral renal ischemia followed by 6 h of reperfusion. After reperfusion, kidneys and blood were obtained for histopathologic and biochemical evaluation. Results. Melatonin and 1400W had an ameliorative effect on both oxidative and nitrosative stress in the kidneys against renal IR injury in rats. In addition, melatonin significantly reduced elevated nitro-oxidative stress product, restored decreased antioxidant enzymes and attenuated histological alterations when compared with 1400W. Conclusions. Both Melatonin and 1400W were efficient in ameliorating experimental IR injury of the kidneys. Moreover, melatonin was more effective than 1400W possibly through inhibiting iNOS as well as scavenging free oxygen radicals and peroxynitrite. © 2009 Informa UK, Ltd.