Enteral glutamine and/or arginine supplementation have favorable effects on oxidative stress parameters in neonatal rat intestine
Journal of Pediatric Gastroenterology and Nutrition, cilt.49, sa.1, ss.85-89, 2009 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 49 Sayı: 1
- Basım Tarihi: 2009
- Doi Numarası: 10.1097/mpg.0b013e318198cd36
- Dergi Adı: Journal of Pediatric Gastroenterology and Nutrition
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.85-89
- Anahtar Kelimeler: Arginine, Glutamine, Necrotizing enterocolitis, Oxidative stress
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: To investigate and compare the effects of enteral glutamine and arginine supply on lipid peroxidation and antioxidant enzyme levels in the small intestine of healthy breast-fed rats. Materials and Methods: The study comprised 40 newborn Sprague-Dawley rats born to 5 mother rats. Newborn rats were randomly divided into 4 groups. Starting from day 1 until day 21, group I received only breast milk; group II received breast milk and 200mg/kg/day oral glutamine; group III received breast milk and 200 mg/kg/day oral arginine; and group IV received breast milk, 200 mg/kg/day glutamine, and 200 mg/kg/day arginine. Malondialdehyde levels and glutathione peroxidase (GPx) and superoxide dismutase activities were measured. Results: The lowest malondialdehyde levels were found in group II (P = 0.0001). Superoxide dismutase activity was found to be significantly higher in group II than group I (P < 0.001). Of the 4 groups, GPx activity was highest in group IV. GPx activity in group II was significantly higher than in group I (P = 0.001) or group III (P = 0.001). GPx activity was higher in group IV than in group I (P = 0.001) or group III (P = 0.001). Conclusions: Enteral glutamine alone or in the presence of arginine has favorable effects on oxidative stress not only in experimental models of hypoxia-reoxygenation, but also in healthy newborn rats. This suggests that in premature neonates with insufficient oxidative resistance, glutamine and arginine supplementation may help prevent necrotizing enterocolitis. JPGN 49:85-89, 2009. © 2009 by european society for pediatric gastroenterology, Hepatology, and nutrition and north american society for pediatric gastroenterology, Hepatology, and nutrition.