Evaluation of the HGF-1 Cells on Titanium Abutments Fixed by Various Procedures Titanyum Abutment Üzerinde Çeşitli Prosedürlerle Fikse Edilmiş HGF-1 Hücrelerinin Değerlendirilmesi
Journal of Uludag University Medical Faculty, cilt.49, sa.1, ss.23-29, 2023 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 49 Sayı: 1
- Basım Tarihi: 2023
- Doi Numarası: 10.32708/uutfd.1203043
- Dergi Adı: Journal of Uludag University Medical Faculty
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.23-29
- Anahtar Kelimeler: Fixation, HGF-1, Immunoflorescence, Ti6Al4V
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
The aim of this study was to compare the effects of fixation protocols in terms of attachment and proliferation of human gingival fibroblasts (HGF-1) on titanium abutment surfaces. HGF-1 cells were seeded on Titanium alloy (Ti6Al4V) plates with dimensions of 10x10x1 cm3. Cells were evaluated at 48 hours. 8 groups were determined (n=4). The specimens were treated with glutaraldehyde (GA) for 30,45 and 60 minutes (Group GA30, GA45, GA60), with formaldehyde (FA) for 6, 12, and 24 hours (Group FA6,FA12,FA24) and with paraformaldehyde (PFA) for 2 hours and 20 minutes (Group PFA2, PFA20) for fixation. Specimens were evaluated under Scanning Electron Microscope (SEM) (n=2) and under Immonoflorescence (n=2). The contact areas of cells in all groups were measured and differences between hours was evaluated statistically. According to SEM images, HGF-1 cell were similar for FA groups as spindled and homogeneous. Cell contact area was 95.83% for FA12 which exhibited the best cell spread among all groups. In GA and PFA groups the cells were elongated, clustered in certain areas and layered with distinct nucleated. According to immunofluorescence images, the densities of actin filaments were observed at similar levels in cells in all three groups, and it was determined that the cell bodies on the titanium surface in the PFA groups were more prominent, and better spread. The fixation process is one of the most critical step of imaging in cell studies. Researchers are needed to consider the most appropriate fixation method in order to obtain successful image results.