Investigation of phenotypic and genotypic characteristics of Acinetobacter baumannii isolated from clinical samples Klinik örneklerden izole edilen Acinetobacter baumannii«nin fenotipik ve genotipik özelliklerinin araştırılması
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.82, sa.2, ss.209-230, 2025 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 82 Sayı: 2
- Basım Tarihi: 2025
- Doi Numarası: 10.5505/turkhijyen.2025.94752
- Dergi Adı: Turk Hijyen ve Deneysel Biyoloji Dergisi
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.209-230
- Anahtar Kelimeler: Acinetobacter baumannii, Acinetobacter baumannii, antibiotic resistance, antibiyotik direnci, biofilm, biyofilm, plasmid, plazmid, REP-PCR, REP-PCR
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: Acinetobacter baumannii strains are common nosocomial pathogens worldwide. Our study aimed to determine the antibiotic resistance rate, blaOXA and ISAba1 genes, metallo-beta-lactamases production, biofilm formation, and clonal classification of A. baumannii isolated from clinical samples in Çorum Erol Olçok Training and Research Hospital, Türkiye. It was also aimed to describe the plasmid profile and analyze the association between genes, clones, and plasmids.Methods: Ninety-eight A. baumannii isolated between 2018 and 2019 were included in the study. Antibiotic susceptibility tests were determined with Vitek 2. The reference broth microdilution method was used to assess colistin susceptibility. These results were compared with those obtained from the Vitek 2. A multiplex polymerase chain reaction detected blaOXA-23>, blaOXA-24/40>, blaOXA-51>, and blaOXA-58> genes. The ISAba1/ blaOXA-23> and ISAba1/ blaOXA-51> genes were analyzed separately via PCR. Genotypes and subtypes of the isolates were determined with the Repetitive Extragenic Palindromic PCR method. International clones were investigated by multiplex PCR. The plasmid profiles of the isolates were analyzed using alkaline lysis. Phenotypic methods were preferred for determining biofilm formation and metallo-beta-lactamase production.Results: A. baumannii was identified in deep tracheal aspirate samples (32%), wounds (21%), blood (17%), sputum (15%), urine (9%), tissue biopsy samples (4%), pleural fluid (1%), and cerebrospinal fluid (1%). A. baumannii was detected in patient samples from the intensive care unit at a frequency of 58%. Of these isolates, 13% were susceptible to all antibiotics tested, while 45% were found to be extensively drug resistant. The ISAba1/blaOXA-23>, ISab1/blaOXA-51>, blaOXA-23>, and blaOXA-51> genes were found in 98.8% of the carbapenemresistant isolates. BlaOXA-24/40 and blaOXA-58 genes were not detected. The epidemiological distribution of the isolates revealed seven genotypes and 21 subtypes. Genotype D was found, and international clone 2 was classified in the hospital epidemic isolates. It was shown that 73.4% of isolates carried plasmids, which were not identical among isolates. Different biofilm levels were measured in 88% of the isolates. However, more vigorous biofilm formation was detected in isolates sensitive to ceftazidime, imipenem, meropenem, trimethoprim-sulfamethoxazole, and ciprofloxacin.Conclusion: The blaOXA-23> genotype was associated with carbapenem resistance in A. baumannii isolates. Genotype D and international clone 2 were defined as endemic isolates in our hospital, and no similarity was found between susceptible and resistant isolates in terms of plasmid profiles, genotypes, and clonality. Stronger biofilm formation was detected in isolates susceptible to ceftazidime, imipenem, meropenem, trimethoprim-sulfamethoxazole, and ciprofloxacin, and more comprehensive studies are needed on the relationship between biofilm formation and antibiotic resistance.