Investigation of imipenem, ciprofloxacin, gentamicin and co-trimoxazole resistant phenotypes in nosocomial Escherichia coli strains Hastane kökenli Escherichia coli suşlarında i̇mipenem, siprofloksasin, gentamisin ve ko-trimoksazol direnç fenotiplerinin araştırılması
Turkiye Klinikleri Journal of Medical Sciences, cilt.31, sa.1, ss.39-44, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 31 Sayı: 1
- Basım Tarihi: 2011
- Doi Numarası: 10.5336/medsci.2009-15762
- Dergi Adı: Turkiye Klinikleri Journal of Medical Sciences
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.39-44
- Anahtar Kelimeler: Drug resistance, bacterial, Escherichia coli, imipenem, ciprofloxacin, gentamicins, trimethoprim-sulfamethoxazole combination, drug resistance, multiple
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: The nosocomial extended spectrum beta-lactamase (ESBL) producing strains may have high and multiple antibiotic resistance features. This resistance may result in clinical problems especially when choice of appropriate antibiotics is limited in life threatening infections. Current study aims to investigate the antibiotic resistance profiles of imipenem, ciprofloxacin, gentamicin and co-trimoxazole in Escherichia coli strains that isolated in our hospital. Material and Methods: Total 974 E.coli strains that were considered as an infectious agent and isolated from clinical samples of hospitalized patients between 2006 and 2008 in our tertiary health care center was included into study. Antibiotic susceptibility test was performed with disc diffusion method according to provisions of Clinical and Laboratory Standarts Institute (CLSI). Double disc synergy test has been utilized for GSBL production. The E.coli strains that were isolated from different clinical samples of same patient with same susceptibility pattern were not included into study. Results: Of all strains examined in three-year period, 53.5% and 51.8% were resistant to co-trimoxazole and ciprofloxacin, respectively. ESBL production was detected in 205 (21%) strains. Co-trimoxazole, ciprofloxacin and gentamicin resistance was significantly higher in ESBL producer E. coli strains (p< 0.001). In 2007 and 2008, gentamicin resistance was 27.8% and 35.5% and ESBL production was 18% and 27%, respectively, and this increase was statistically significant. Imipenem resistance was detected in five strains. Multiple drug resistance was detected in 19.2% and co-resistance phenotype in 45.5%. Conclusion: We concluded that in our study imipenem was the most efficient antibiotic and resistance to co-tri- moxazole, ciprofloxacin and gentamicin was common especially among ESBL producer E.coli strains. In our region, empirical treatment of infections with these antibiotics might be insufficient for nosocomial E.coli strains. © 2011 by Türkiye Klinikleri.