Protective effects of colchicine in an experimental model of necrotizing enterocolitis in neonatal rats
Journal of Surgical Research, cilt.183, sa.1, ss.156-162, 2013 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 183 Sayı: 1
- Basım Tarihi: 2013
- Doi Numarası: 10.1016/j.jss.2013.01.002
- Dergi Adı: Journal of Surgical Research
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.156-162
- Anahtar Kelimeler: Necrotizing enterocolitis, Colchicine, Tumor necrosis factor-alpha, Interleukin-1 beta
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Background: The pathophysiology of necrotizing enterocolitis (NEC) includes the massive production of endogenous cytokines with exaggerated activation of inflammatory pathways. Colchicine has been used as an anti-inflammatory agent. The aim of this study was to investigate the possible beneficial effects of colchicine in a neonatal rat model of NEC. Materials and methods: We randomly divided rat pups into three groups: a control group, a saline-treated NEC group, and a colchicine-treated NEC group. We induced NEC by hyperosmolar enteral formula feeding and exposure to hypoxia/reoxygenation after cold stress. Intestinal samples were harvested for biochemical and histopathologic analyses. Results: The grade of intestinal injury of pups in the saline-treated NEC group was significantly higher than in the control and colchicine-treated groups (P < 0.001 and 0.003, respectively). The median level of intestinal malondialdehyde was significantly higher in the saline-treated NEC group compared with the control group (P = 0.006) or the colchicine-treated NEC group (P = 0.015). We observed significantly higher activity levels of intestinal superoxide dismutase and glutathione peroxidase in the colchicine-treated NEC group compared with the saline-treated NEC group (P = 0.033 and 0.030, respectively). The tissue levels of tumor necrosis factor-α and interleukin-1β were significantly higher in the saline-treated NEC group compared with the colchicine-treated NEC group (P < 0.001 and 0.003, respectively). Conclusions: We observed that in this model of NEC, colchicine had favorable effects on intestinal histologic and biochemical changes. © 2013 Elsevier Inc. All rights reserved.