IL-10 and IL-13 production by peripheral blood mononuclear cells in patients with diabetes mellitus
Turkish Journal of Medical Sciences, cilt.38, sa.1, ss.43-48, 2008 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 38 Sayı: 1
- Basım Tarihi: 2008
- Dergi Adı: Turkish Journal of Medical Sciences
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.43-48
- Anahtar Kelimeler: IL-10, IL-13, diabetes mellitus
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Aim: It is generally accepted that proinflammatory cytokines secreted by mononuclear cells are responsible for pancreatic beta (B) cell destruction in animal models of autoimmune type-1 diabetes mellitus (DM). Several studies have shown that markers of inflammation and pro-inflammatory cytokines such as interleukin (IL)-10 and IL-13 associate with the metabolic syndrome, dyslipidemia, type-2 DM and type-1 DM. The aim of this study was to examine the in vitro production of IL-10 and IL-13 in cultures of peripheral lymphocytes obtained from patients with type-2 DM, type-1 DM and healthy controls. Materials and Methods: Twenty patients (10 females, 10 males) with type-2 DM (median disease duration: 10 years), 10 patients (5 females, 5 males) with type-1 DM (median disease duration: 11 years) and the healthy control group were enrolled in the study. IL-10 and IL-13 in culture supernatants were measured using the enzyme immunoassay method. Statistical analyses were performed using Mann-Whitney U test, Wilcoxon signed rank tests, and Spearman's rank correlation. Results: IL-10 and IL-13 levels were similar in culture supernatants incubated with 10 μg/ml phytohemagglutinin (PHA) in all patients and healthy controls (P > 0.05). In type-2 DM, IL-10 levels were significantly lower than in the control group in culture supernatants incubated with 20 μg/ml PHA (P = 0.001 for poor metabolic control and P = 0.048 for good metabolic control, respectively). Conclusions: According to this study, the alterations in IL-13 did not play an important role in the pathogenesis of type-2 DM and type-1 DM. Furthermore, the deficiency in IL-10 production from peripheral blood mononuclear cells observed with high dosages of PHA suggests that IL-10 may play an important role in the pathogenesis of type-2 DM. © TÜBİTAK.