The modulation of glucocorticoid receptor content by 3-O-methyl-D-glucose transport in human mononuclear leukocyte in obesity
Journal of Endocrinological Investigation, cilt.21, sa.10, ss.656-661, 1998 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 21 Sayı: 10
- Basım Tarihi: 1998
- Doi Numarası: 10.1007/bf03350794
- Dergi Adı: Journal of Endocrinological Investigation
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.656-661
- Anahtar Kelimeler: 3-O-Methyl-D-glucose transport, Cortisol, Cushing's syndrome, Glucocorticoid receptors, Mononuclear leukocyte, Obesity
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Glucocorticoid receptors (GR) and 3-O-methyl-D glucose (3-O-MG) transport were determined in mononuclear leukocytes (MNL) from 11 abdominal obese subjects, 10 pituitary-dependent Cushing's syndrome (Cushing's disease) and 10 healthy controls. Using a whole-cell competitive binding assay and 3H-dexamethasone as tracer, MNL of abdominal obese subjects were found to have 4855 ± 1389 sites/cell which was significantly lower (p < 0.05) than controls (6234 ± 1568 sites/cell), although no significant difference was found in the mean serum cortisol level. Their mean Kd (affinity) was also significantly lower than that found in the healthy controls (obese Kd:2.92 ± 0.84 nmol/l, control Kd: 4.55 ± 0.67 nM, p < 0.05). On the other hand, the receptor characteristics in Cushing's disease patients were within the normal range. At the same time, 3-O-MG transport was determined in the same subjects. In Cushing's disease, 3-O-MG transport was within the normal range, whereas in abdominal obesity this value was significantly lower than the healthy controls (abdominal obese: 31.90 ± 8.20; control: 46.26 ± 12.91 fmol/106 cell. min, p < 0.05). We also found a positive correlation between 3-O-MG transport and GR binding capacity in abdominal subjects (r = 0.89, p < 0.001), however we did not find such a correlation in Cushing's disease (r = 0.60, p > 0.05). These results indicated that, in abdominal obesity, the GR binding capacity in MNL is influenced by the changes in glucose transport.