Macrolide-lincosamide-streptogramin B resistant phenotypes and genotypes for methicillin-resistant staphylococcus aureus in Turkey, from 2003 to 2006
Polish Journal of Microbiology, cilt.57, sa.4, ss.307-312, 2008 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 57 Sayı: 4
- Basım Tarihi: 2008
- Dergi Adı: Polish Journal of Microbiology
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.307-312
- Anahtar Kelimeler: MLSB, MRSA, Multiplex real-time PCR, Staphylococcus aureus
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Methicillin-resistant Staphylococcus aureus (MRSA) strains with inducible macrolide-lincosamide-streptogramin B (iMLSB) resistance phenotype may lead to clinical failure during clindamycin (CLI) therapy. The aim of this study was to determine the incidence of MLSB phenotypes by using D-test method and genotypes by using multiplex real-time PCR method in MRSA strains. A total of 265 MRSA strains were obtained from clinical samples from hospitalized and outpatients. Of the MRSA isolates, 225 (84.9%) were resistant to erythromycin (ERT), and 170 (64.1%) to CLI. Among the 225 ERT-resistant MRSA strains, the constitutive MLSB (cMLSB) rate was found in 49.3%, iMLSB in 39.1% and the M phenotype in 11.5%. Overall, ermA, ermC, ermA+ermC, msrA, ermC+msrA, and ermA+ermC+msrA genes were detected in 85 (37.7%), 60 (26.6%), 42 (18.6%), 26 (11.5%), 11 (4.8%), and 1 (0.4%) isolates, respectively. Most prevalent resistance determinant in MRSA strains was ermA which was detected in 37.7% of the isolates. The 26 MRSA strains with M phenotype harboured only msrA gene. In conclusion, due to aware of the potential of CLI treatment failure, D-test should be performed and reported in MRSA strains in clinical laboratories. The multiplex real-time PCR method is easy to perform, fast and reliable method for the detection of MLSB resistance genotypes.