Calcium Butyrate-Integrated Bioadhesive Hydrogel: a Promising Therapeutic Platform for Inflammation Control and Wound Regeneration
Journal of Pharmaceutical Innovation, cilt.21, sa.2, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 21 Sayı: 2
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s12247-026-10425-0
- Dergi Adı: Journal of Pharmaceutical Innovation
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, INSPEC, Health Research Premium Collection (ProQuest)
- Anahtar Kelimeler: Butyric acid, Calcium butyrate, Bioadhesive gel, Rheology, Anti-inflammatory, Wound healing
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Purpose: Butyric acid (a short-chain fatty acid) has anti-inflammatory and wound-healing potential but its direct use is limited by instability, odor, and irritancy. This study asked whether loading calcium butyrate (CAB) into a bioadhesive hydrogel suited for dermal delivery would yield acceptable physicochemical performance, sustained release, cytocompatibility, anti-inflammatory activity, pro-migratory/wound-closure effects, and non-irritancy. Methods: Hydrogels were prepared with carboxymethyl cellulose, xanthan gum, glycerin, and rose water, generating a blank matrix (F1) and a CAB-loaded system (F1-CAB). Formulations were characterized for pH, viscosity, CAB conten in the formulationt, texture/spreadability, rheology/thermal behavior, and in-vitro release kinetics. Biological evaluations followed ISO 10,993 principles using L929 fibroblasts, RAW 264.7 macrophages, and reconstructed human epidermis models. Endpoints included cell viability (cytotoxicity), nitrite inhibition (anti-inflammatory activity) versus CAB alone, fibroblast migration/wound-closure assays, and in-vitro irritation (tissue viability). Results: CAB incorporation increased viscosity, bioadhesion, and viscoelastic strength. F1-CAB exhibited shear-thinning behavior and superior thermal stability, consistent with Ca²⁺-mediated crosslinking. CAB release was sustained. The formulation showed excellent cytocompatibility with viability > 70% at all concentrations tested. At 1 mg/mL, F1-CAB produced 34% greater nitrite inhibition than CAB alone. F1-CAB enhanced fibroblast migration in a dose-dependent manner, accelerating wound closure. Reconstructed epidermis viability remained > 80%, indicating non-irritant properties. Conclusion: A CAB-loaded bioadhesive hydrogel achieved favorable physicochemical/mechanical profiles, sustained release, robust anti-inflammatory effects, and pro-healing activity while maintaining biocompatibility and non-irritancy. These findings support CAB hydrogels as a promising dermal platform for inflammatory skin conditions and regenerative applications, meriting further preclinical development.