Evaluationofagmatineefficacyincontrast-inducednephropathy:experimental evidence in rat model


Yeniçeri M., BAŞ S., Şenoymak M. C., SALMANOĞLU M., Tanoğlu A.

Acta Cirurgica Brasileira, cilt.41, 2026 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41
  • Basım Tarihi: 2026
  • Doi Numarası: 10.1590/acb412426
  • Dergi Adı: Acta Cirurgica Brasileira
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE, Directory of Open Access Journals
  • Anahtar Kelimeler: Agmatine, Iohexol, Contrast Media
  • Sağlık Bilimleri Üniversitesi Adresli: Evet

Özet

Purpose: To investigate the biochemical and histopathological efficacy of agmatine as a protective agent in experimental rat contrast-induced nephropathy (CIN) model. Methods: Twenty-eight male Sprague Dawley rats were randomized into four groups: sham (no intervention), contrast agent (single dose 10 mL/kg iohexol iv), contrast agent + agmatine (single dose 10 mL/kg iohexol iv + 20 mg/kg po agmatine three days) and contrast agent + placebo (single dose 10 mL/kg iohexol iv + 1 mL of 0.9% saline ip). Biochemical and histopathological evaluations were accomplished on the fourth day, including measurements of cystatin-C, malondialdehyde (MDA), glutathione peroxidase (GPx), interleukin (IL)-1 β, superoxide dismutase (SOD), and tumor necrosis factor (TNF)-α. Results: The contrast agent group exhibited elevated cystatin-C, IL-1β, TNF-α, and MDA levels, with decreased SOD and GPx levels. The agmatine treatment group showed decrease in cystatin-C, IL-1β, TNF-α, and MDA, along with increased GPx and SOD levels. Histopathological evaluation revealed nephrotoxic effects in the contrast agent and placebo groups, whereas agmatine-treated rats displayed a significant reduction in tubular damage. Conclusion: Agmatine administration along side contrast exposure demonstrated protective effects against CIN, evidenced by biochemical and histopathological improvements.