Use of Mindray MC-80 digital morphology analyzer's estimated platelet counts as adjunct to automated hematology analyzer
Acta Haematologica Polonica, cilt.54, sa.3, ss.169-175, 2023 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 54 Sayı: 3
- Basım Tarihi: 2023
- Doi Numarası: 10.5603/ahp.a2023.0025
- Dergi Adı: Acta Haematologica Polonica
- Derginin Tarandığı İndeksler: Scopus, BIOSIS, Central & Eastern European Academic Source (CEEAS), EMBASE
- Sayfa Sayıları: ss.169-175
- Anahtar Kelimeler: method comparison, platelet estimation, digital morphology analyzer, thrombocytopenia, auto analyzer
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Introduction: Automated hematology analyzer platelet counts can be performed using either the impedance (PLT-I) method or the fluorescent nucleic acid staining (PLT-O) method. Estimated platelet counts (PLT-E) can be calculated using a digital morphology analyzer by evaluating the peripheral blood smear. Our objective was to compare the platelet values detected on a Mindray BC-6200 device to the PLT-E count on a Mindray MC-80 digital morphology analyzer. Material and methods: Complete blood cell count findings between 1 September and 11 October, 2022 were obtained from the data storage units of the devices. We selected two groups of blood: a first group with thrombocytopenia (n = 49) and a second group that gave an aggregation and/or platelet clumping flag (n = 32). The results of 190 consecutive patients with normal platelet counts, and no aggregation flag, were evaluated as a control group. Pearson's correlation coefficients, Bland-Altman plots, and paired t-tests were calculated. Results: The plot of the difference between PLT-I and PLT-O counts showed that the mean difference was -43.6 (95%: -17.2 to -69.9); when we compared PLT-O to PLT-E, bias was improved to -6.1 (95%: -18.26 to 6.1) in samples with aggregation and/or clumping flags (Bland-Altman plots). In samples with thrombocytopenia without aggregation and/or clumping, on the Bland-Altman plot, the differences in means were all close to zero, and there were no definite biases. Conclusions: Examining blood samples using the Mindray MC-80 digital morphology analyzer system on samples that show platelet clumps has the potential to improve PLT-I results in day-to-day laboratory routine.