Investigations on effects of titanium dioxide (TiO2) nanoparticle in combination with UV radiation on breast and skin cancer cells
Medical Oncology, cilt.40, sa.1, 2023 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 40 Sayı: 1
- Basım Tarihi: 2023
- Doi Numarası: 10.1007/s12032-022-01931-5
- Dergi Adı: Medical Oncology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CINAHL, MEDLINE
- Anahtar Kelimeler: Titanium dioxide nanoparticle, Ultraviolet radiation, Breast cancer, Skin cancer
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
In this study, we have investigated the chemotherapeutic potential of titanium dioxide (TiO2) nanoparticles on skin and breast cancer cells. The cells have treated with a 75 µg/ml concentration of titanium dioxide because it is a recommended dose with proven effectiveness in vitro studies and then the cells were exposed to UV-A radiation. The combined effects of titanium dioxide and UV-A radiation on cell viability, cell cycle, plasma membrane, mitochondrial membrane potentials and apoptotic activity of the cells were investigated. The viability of SK-MEL 30 cells was measured by MTT assay and apoptotic activity of cells was determined by Annexin-V FITC/PI staining. As a result of the research, an increase was observed in the viability of cells treated with 75 µg/ml titanium dioxide concentration, while a significant decrease in cell viability was observed for both cell types when UV-A radiation and TiO2 were applied together. The results also showed that the percentage of apoptotic cells increased as a result of UV + TiO2 exposure. Accordingly, it can be said that TiO2 nanoparticles may research as potential chemotherapeutic agents for skin and breast cancers, especially in the presence of UV radiation.