A recombinant humanized monoclonal antibody for treatment of endometriosis in a rat model


Işik H., MORALOĞLU TEKİN Ö., Kilic S., Seven A., Caydere M., Batioǧlu S.

Journal of Endometriosis, cilt.3, sa.3, ss.143-150, 2011 (Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 3 Sayı: 3
  • Basım Tarihi: 2011
  • Doi Numarası: 10.5301/je.2011.8724
  • Dergi Adı: Journal of Endometriosis
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.143-150
  • Anahtar Kelimeler: Anti-VEGF, Bevacizumab, Endometriosis, Rat model
  • Sağlık Bilimleri Üniversitesi Adresli: Evet

Özet

Aim: Angiogenesis plays an important role in the pathogenesis of endometriosis. Thus, the inhibition of angiogenesis may prevent endometriosis. Bevacizumab is a recombinant humanized monoclonal antibody against VEGF. The aim of this study is to investigate the efficacy of anti-VEGF therapy on the prophylaxis and treatment of endometriotic foci in a rat model. Methods: This experimental study is prospective, randomized, and placebo-controlled. Thirty-six Wistar- Albino female rats were divided into 3 groups. Experimental endometriosis was induced by the implantation of autologous endometrial tissue. The bevacizumab administration route was intraperitoneal. Group A was the prophylaxis group; Group B was the treatment group, and Group C was the control group. The volumes of the implants as well as their VEGF and Ki-67 immunohistochemical staining are main outcome measures. Results: The volumes of the lesions were smaller in Group A than Group C [P<.05]. The volumes of endometriotic foci in Group B were smaller than in Group C [P<.05]. Bevacizumab caused regression and atrophy of the endometriotic lesions. After the treatment the histopathologic and immunohistochemical scores in Group B were less than before treatment and less than the scores in Group C. Conclusions: Bevacizumab treatment had a regressive effect on the endometriotic implants. As an anti- VEGF agent, bevacizumab has beneficial effects on the prophylaxis and treatment of endometriosis. © 2011 Wichtig Editore.