The isolated bacteria from culture and antibiotic susceptibilities in urinary tract infections I˙drar yolu enfeksiyonlarinda kültürden izole edilen bakteriler ve antibiyotik duyarliliklari
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.77, sa.1, ss.25-32, 2020 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 77 Sayı: 1
- Basım Tarihi: 2020
- Doi Numarası: 10.5505/turkhijyen.2019.57984
- Dergi Adı: Turk Hijyen ve Deneysel Biyoloji Dergisi
- Derginin Tarandığı İndeksler: Scopus, Academic Search Premier, CAB Abstracts, Veterinary Science Database
- Sayfa Sayıları: ss.25-32
- Anahtar Kelimeler: Antibiotic susceptibility, Urinary tract infection, Urine culture
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: Urinary tract infection is the second most common among all infectious diseases. Urinary tract infection is defined as presence of bacteriuria and/ or pyuria with clinical findings such as costovertebral angle sensitivity, fever, suprapubic tenderness, dysuria, pollacuria and urinary incontinence. The most commonly isolated bacterium in acute infections is Escherichia coli. The aim of this study is to determine the bacteria isolated from the urine cultures of patients with urinary tract infections and to investigate the antibiogram results of these bacteria and to investigate their susceptibility and resistance to antibiotics commonly used in treatment. Methods: In this study, urine cultures sent to the clinical microbiology laboratory of a tertiary hospital between December 1, 2014 and October 1, 2016 with the diagnosis of urinary tract infection were retrospectively analyzed. Patients diagnosed with urinary tract infection with pyuria and 105 colony/mL growth in culture in a fully automated urine test were included in the study. Identification of the reproductive factors and antibiotic susceptibility tests were performed by disc diffusion method according to the recommendations of The European Committee on Antimicrobial Susceptibility Testing (EUCAST). The data was expressed as numbers and percentages. Results: The urine culture results of 76 patients were evaluated. 48 strains isolated from urine cultures and antibiogram results of these strains were examined. The most frequently isolated microorganism was E. coli (69%) and 33 strains were detected. Expanded spectrum betalactamase (ESBL)-positive E. coli 20 (42%), ESBL-negative E. coli 13 (27%), ESBL-positive Klebsiella pneumoniae 5 (10%) in 48 (100%) strains isolated and ESBL-negative K. pneumoniae 2 (4%), other gram negative and positive bacteria were detected as 8 (17%) strains. Proteus mirabilis, Enterococcus faecalis, Enterococcus faecium, Pseudomonas aeruginosa, Streptococcus agalactiae, Stenotrophomonas maltophilia and Acinetobacter lwoffii have been identified as other factors. Sensitivity of imipenem, meropenem, cefoperazone-sulbactam, piperacillin-tazobactam, cefepime, ciprofloxacin, amikacin and nitrofurantoin was 100% and tobramycin was 92.31% in ESBL-negative E. coli. Conclusion: ESBL-positive E. coli was detected at 42%. This rate supports the increasing antibiotic resistance results over the years. The resistance to ciprofloxacin, trimethoprim-sulfamethoxazole and cefuroxime, which are often preferred in the treatment of empirical antibiotics, increases. Therefore, empirical antibiotic treatment selection should be revised in patients with urinary tract infections.