Verification of enzymatic ethanol analysis method and method comparison with headspace gas chromatography

Ozturk A., Temel I., Yalcindag A., Ucar F., Yay F.

Turkish Journal of Biochemistry, cilt.47, sa.5, ss.557-563, 2022 (SCI-Expanded, Scopus, TRDizin)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 47 Sayı: 5
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1515/tjb-2021-0280
  • Dergi Adı: Turkish Journal of Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, Food Science & Technology Abstracts, Directory of Open Access Journals, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.557-563
  • Anahtar Kelimeler: enzymatic method, ethanol, headspace gas chromatography, method comparison, method verification, enzimatik metot, etanol, headspace gaz kromatografisi, metot karsilastirma, metot verifikasyonu
  • Sağlık Bilimleri Üniversitesi Adresli: Evet

Özet

Objectives: This study aimed to verify the enzymatic ethanol analysis method and compare the technique with headspace gas chromatography (HS/GC). Methods: The limit of the blank, limit of detection, and limit of quantification, precision, linearity, and accuracy of the enzymatic process were evaluated based on the data obtained. In addition, a method comparison was made between the enzymatic method and the HS/GC analysis method. Results: It was seen that the enzymatic method was linear in the range of 14-450 mg/dL, the coefficient of variation and bias values ranged between 0.5-1.09% and 4.52-9.78%, respectively. The quantitative accuracy was 2.13% and 2.23% at concentrations of 41.16 and 95.82 mg/dL, respectively. When the regression analysis was performed between the results obtained using the enzymatic alcohol dehydrogenase (ADH) and HS/GC methods, y=0.966x + 2.084 linear relationship formula and regression coefficient of r2=0.9987, it was found that it showed a good agreement at a confidence interval of 5%. Conclusions: The findings showed that the enzymatic method had good linearity, excellent precision, and a high correlation with the higher-order comparator method, the HS/GC method, in the ethanol analysis.