Prognostic value of expression levels of miR‑148a, miR‑152 and HLA‑G in colon cancer
Oncology Letters, cilt.24, sa.1, 2022 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 24 Sayı: 1
- Basım Tarihi: 2022
- Doi Numarası: 10.3892/ol.2022.13347
- Dergi Adı: Oncology Letters
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, EMBASE
- Anahtar Kelimeler: colon cancer, human leukocyte antigen G, microRNA-148a, microRNA-152, survival
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Aberrant expression of human leukocyte antigen G (HLA‑G), an immunosuppressive molecule, has been observed in various cancer types. The exact mechanism of HLA‑G expression is unclear. HLA‑G expression is associated with low expression levels of microRNA (miR)‑148a and miR‑152. To the best of our knowledge, the prognostic value of the expres‑ sion levels of miR‑148a, miR‑152 and HLA‑G has not been investigated in colon cancer. The aim of the present study was to investigate the relationship between the presence of HLA‑G molecules and the expression levels of miR‑148 and miR‑152 in colon cancer. In addition, the association of both HLA‑G and miR expression with survival results and clinicopathological data was determined. An immunohistochemical method was used for detection of HLA‑G expression in the tumor tissues and adjacent healthy tissues of 108 patients with colon cancer. Reverse transcription‑quantitative PCR was used for miR analysis. HLA‑G was expressed in 82 (75.9%) of the cancer samples, and there was no staining in normal tissues. HLA‑G expression >20%, which was found in 41.7% (45/108) of the patients with colon cancer, was significantly positively associ‑ ated with patient survival (P=0.039). Additionally, miR‑148a levels were lower in the tumor tissues compared with the adja‑ cent healthy tissues. No differences were found for miR‑152 expression. In addition, mir‑148a levels were found to be significantly lower (P=0.001) in HLA‑G‑positive tumor tissues than HLA‑G‑negative tumor tissues, but no such relationship was found for miR‑152 levels. The presence of HLA‑G expres‑ sion was associated with poor survival outcomes. HLA‑G is one of the prognostic factors in colon cancer, and decreased.