Effect of Bumetanide on Facial Nerve Regeneration in Rat Model
Otolaryngology - Head and Neck Surgery (United States), cilt.164, sa.1, ss.117-123, 2021 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 164 Sayı: 1
- Basım Tarihi: 2021
- Doi Numarası: 10.1177/0194599820937670
- Dergi Adı: Otolaryngology - Head and Neck Surgery (United States)
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, CINAHL, EMBASE, MEDLINE, Veterinary Science Database
- Sayfa Sayıları: ss.117-123
- Anahtar Kelimeler: aquaporin, bumetanide, facial paralysis, regeneration
- Sağlık Bilimleri Üniversitesi Adresli: Evet
Özet
Objective: We investigated the effects of bumetanide alone and in combination with dexamethasone on facial nerve regeneration in rats with facial paralysis. Study Design: A prospective controlled animal study. Setting: An animal laboratory. Subjects and Methods: Facial paralysis was induced in 32 Wistar rats that we then divided into 4 groups: group 1, control; group 2, bumetanide; group 3, dexamethasone; group 4, bumetanide and dexamethasone. Electroneurography was performed 1, 2, and 4 weeks later, and nerve regeneration was evaluated by electron and light microscopy and Western blotting in week 4. Results: Regarding the comparison between preoperative values and week 4, the latency difference in group 1 (1.25 milliseconds) was significantly higher than those of groups 2 to 4 (0.56, 0.34, and 0.10 milliseconds, respectively; P =.001). The latency increment in groups 2 and 3 was higher than that of group 4 (P =.002 and P =.046) in week 4, whereas groups 2 and 3 did not differ significantly (P =.291). Amplitude difference was not statistically significant from week 4 among all groups (all P >.05). The number of myelinated axons was significantly higher in all treatment groups than in the control group (P =.001). Axon number and intensity were significantly higher in group 4 as compared with groups 2 and 3 (P =.009, P =.005). Conclusion: After primary neurorrhaphy, dexamethasone and bumetanide alone promoted nerve recovery based on electrophysiologic and histologic measures. Combination therapy was, however, superior.